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Reader Comments (0) Figures. The effects of long-term (over several years) anabolic androgen steroids (AAS) administration on human skeletal muscle are still unclear. In this study, seventeen strength training athletes were recruited and individually interviewed regarding self-administration of banned substances. Ten subjects admitted having taken AAS or AAS derivatives for the past 5 to 15 years (Doped) and the dosage and type of banned substances were recorded. The remaining seven subjects testified to having never used any banned substances (Clean). For all subjects, maximal muscle strength and body composition were tested, and biopsies from the vastus lateralis muscle were obtained. Using histochemistry and immunohistochemistry (IHC), muscle biopsies were evaluated for morphology including fiber type composition, fiber size, capillary variables and myonuclei. Compared with the Clean athletes, the Doped athletes had significantly higher lean leg mass, capillary per fibre and myonuclei per fiber. In contrast, the Doped athletes had significantly lower absolute value in maximal squat force and relative values in maximal squat force (relative to lean body mass, to lean leg mass and to muscle fiber area). Using multivariate statistics, an orthogonal projection of latent structure discriminant analysis (OPLS-DA) model was established, in which the maximal squat force relative to muscle mass and the maximal squat force relative to fiber area, together with capillary density and nuclei density were the most important variables for separating Doped from the Clean athletes (regression = 0. Self-reported intake of banned substances in the Doped group. All the subjects reported that they had trained regularly between 4’6 times per week for at least five years. The physical training was defined as self-reported mean hours of exercise training each week during the past five years, and consisted mainly of high intensity resistance training. The Doped group consists of a mixture of bodybuilders, strongmen competitors and weightlifters whereas the Clean group consists of weightlifters only. The mode of resistance training differs slightly between the two groups; the Doped group used both 1’4 repetitions/set and 8’12 repetitions/set, while the Clean group used mainly 1’4 repetitions/set. We have to emphasize that this is the only ethically feasible approach to study long term effects of AAS abuse on athletes. Muscle strength and body composition, mgf 2 mg peptides $14.00. Subjects performed static squats at a 105′ knee angle in a custom-made Smith squat machine and ground reaction forces were recorded by AMTI force plates (464 ? 508 mm, Advanced Mechanical Technology Incorporated, Massachusetts, USA). Forces were recorded in x, y and z directions at 100 Hz using the Qualisys Track Manager (QTM) software (Qualisys AB, Gothenburg, Sweden). Maximal force (one single recording), Mean of the highest 50 recordings and Mean of 0. Personal records (PR) from competition (without tight suits) or equivalent (not all participants had competed in all disciplines) for Bench press, Squat lift and Deadlift were also used for comparisons. Blood sample of 10 ml was collected from all subjects the same time in the morning after overnight fasting by venipuncture from the cubital vein. Because we could not perform regular doping tests on the subjects and the Doped subjects were not on a ‘cycle’, indirect indicator of blood hormone level was used to prove/disprove the use of AAS. Skeletal muscle biopsies were obtained from the vastus lateralis muscle using standard needle or forceps biopsy technique [18], [19]. The biopsies were taken from the middle part of the muscle, mounted in OCT compound (Tissue Tek, Miles laboratories, Naperville, IL, USA) and then frozen in liquid propane chilled with liquid nitrogen and stored at ?80’C until further processing.
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